@article{39,
  author = {Sergei Filichkin and Jason Cumbie and Palitha Dharmawardhana and Pankaj Jaiswal and Jeff Chang and Saiprasad Palusa and A Reddy and Molly Megraw and Todd Mockler},
  title = {Environmental stresses modulate abundance and timing of alternatively spliced circadian transcripts in Arabidopsis.},
  abstract = {Environmental stresses profoundly altered accumulation of nonsense mRNAs including intron-retaining (IR) transcripts in Arabidopsis. Temporal patterns of stress-induced IR mRNAs were dissected using both oscillating and non-oscillating transcripts. Broad-range thermal cycles triggered a sharp increase in the long IR CCA1 isoforms and altered their phasing to different times of day. Both abiotic and biotic stresses such as drought or Pseudomonas syringae infection induced a similar increase. Thermal stress induced a time delay in accumulation of CCA1 I4Rb transcripts, whereas functional mRNA showed steady oscillations. Our data favor a hypothesis that stress-induced instabilities of the central oscillator can be in part compensated through fluctuations in abundance and out-of-phase oscillations of CCA1 IR transcripts. Taken together, our results support a concept that mRNA abundance can be modulated through altering ratios between functional and nonsense/IR transcripts. SR45 protein specifically bound to the retained CCA1 intron in vitro, suggesting that this splicing factor could be involved in regulation of intron retention. Transcriptomes of nonsense-mediated mRNA decay (NMD)-impaired and heat-stressed plants shared a set of retained introns associated with stress- and defense-inducible transcripts. Constitutive activation of certain stress response networks in an NMD mutant could be linked to disequilibrium between functional and nonsense mRNAs.},
  year = {2015},
  journal = {Molecular plant},
  volume = {8},
  pages = {207-27},
  month = {2015 Feb},
  issn = {1752-9867},
  doi = {10.1016/j.molp.2014.10.011},
  language = {eng},
}