02637nas a2200433   4500000000100000000000100001008004100002260001300043653002400056653001500080653001700095653001700112653002100129653003300150653002100183653003800204653001700242653001700259653001900276653003000295653002200325653001700347653002600364653002500390653002700415653001300442100002100455700002700476700001900503700001900522700002200541700001600563700001700579245010900596300001200705490000700717520146500724022001402189       2002                            d  c2002 Jul10aAmino Acid Sequence10aAntibodies10aCell Nucleus10aChloroplasts10aDNA, Chloroplast10aDNA-Directed RNA Polymerases10aEscherichia coli10aGene Expression Regulation, Plant10aGenes, Plant10aMitochondria10aPlant Proteins10aPromoter Regions, Genetic10aProtein Transport10aSigma Factor10aSubstrate Specificity10aTerminology as Topic10aTranscription, Genetic10aZea mays1 aThomas Beardslee1 aSanchita Roy-Chowdhury1 aPankaj Jaiswal1 aLaurence Buhot1 aSilva Lerbs-Mache1 aDavid Stern1 aLori Allison00aA nucleus-encoded maize protein with sigma factor activity accumulates in mitochondria and chloroplasts.  a199-2090 v313 aPlants contain nuclear gene families that encode proteins related to the principal sigma factors of eubacteria. As sigma factors function in transcription, the plant proteins have been presumed or demonstrated to associate with the eubacteria-like RNA polymerase of chloroplasts. In maize, five sig cDNA sequences have been reported, and four of the products are present in plastids as predicted. However, in vitro chloroplast import assays and computer algorithms gave ambiguous results with the fifth protein, ZmSig2B. Unlike the other maize sigma factors, ZmSig2B is expressed throughout developing seedling leaves, as well as in roots and etiolated tissues. To determine the subcellular location of ZmSig2B, we have now used immunoblot assays to show that it co-purifies with both mitochondria and plastids. Its NH2-terminal 153 amino acids, translationally fused to green fluorescent protein (GFP), targeted GFP to chloroplasts and mitochondria in bombarded maize leaves. A putative role for ZmSig2B in mitochondrial transcription is supported by its presence in a maize mitochondrial transcription extract. ZmSig2B also exhibits the expected properties of a chloroplast sigma factor: recombinant ZmSig2B binds to a chloroplast promoter and initiates transcription in vitro when combined with Escherichia coli core RNA polymerase. Therefore ZmSig2B is an unusual nucleus-encoded sigma factor that appears to function in both chloroplasts and mitochondria.  a0960-7412