02289nas a2200409   4500000000100000000000100001008004100002260001600043653001300059653003800072653001200110653001300122653001500135653000800150653002600158653001000184100001500194700001500209700001600224700001000240700001400250700001400264700001700278700001600295700001600311700001100327700001300338700001600351700001900367700001900386700001600405245011200421300001200533490000800545520131200553022001401865       2022                            d  c2022 Aug 2910aDroughts10aGene Expression Regulation, Plant10aPopulus10aProteome10aProteomics10aRNA10aStress, Physiological10aXylem1 aYubang Gao1 aXuqing Liu1 aYandong Jin1 aJi Wu1 aShuang Li1 aYaxing Li1 aBinqing Chen1 aYaxin Zhang1 aLinxiao Wei1 aWei Li1 aRuili Li1 aChentao Lin1 aAnireddy Reddy1 aPankaj Jaiswal1 aLianfeng Gu00aDrought induces epitranscriptome and proteome changes in stem-differentiating xylem of Populus trichocarpa.  a459-4790 v1903 a<p>Understanding gene expression and regulation requires insights into RNA transcription, processing, modification, and translation. However, the relationship between the epitranscriptome and the proteome under drought stress remains undetermined in poplar (Populus trichocarpa). In this study, we used Nanopore direct RNA sequencing and tandem mass tag-based proteomic analysis to examine epitranscriptomic and proteomic regulation induced by drought treatment in stem-differentiating xylem (SDX). Our results revealed a decreased full-length read ratio under drought treatment and, especially, a decreased association between transcriptome and proteome changes in response to drought. Epitranscriptome analysis of cellulose- and lignin-related genes revealed an increased N6-Methyladenosine (m6A) ratio, which was accompanied by decreased RNA abundance and translation, under drought stress. Interestingly, usage of the distal poly(A) site increased during drought stress. Finally, we found that transcripts of highly expressed genes tend to have shorter poly(A) tail length (PAL), and drought stress increased the percentage of transcripts with long PAL. These findings provide insights into the interplay among m6A, polyadenylation, PAL, and translation under drought stress in P. trichocarpa SDX.</p>
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