TY - JOUR
KW - Phylogeny
KW - Myosins
KW - Multigene Family
KW - Molecular Sequence Data
KW - Models, Genetic
KW - Genes, Plant
KW - Gene Expression Regulation, Plant
KW - Gene Expression Regulation, Developmental
KW - Gene Expression Profiling
KW - Evolution, Molecular
KW - Circadian Rhythm
KW - Bryophyta
KW - Brachypodium
KW - Arabidopsis
KW - Alternative Splicing
KW - Terminology as Topic
KW - RNA, Messenger
KW - Protein Isoforms
AU - Valera Peremyslov V
AU - Todd Mockler
AU - Sergei Filichkin
AU - Samuel Fox
AU - Pankaj Jaiswal
AU - Kira Makarova
AU - Eugene Koonin V
AU - Valerian Dolja V
AB - Plants possess two myosin classes, VIII and XI. The myosins XI are implicated in organelle transport, filamentous actin organization, and cell and plant growth. Due to the large size of myosin gene families, knowledge of these molecular motors remains patchy. Using deep transcriptome sequencing and bioinformatics, we systematically investigated myosin genes in two model plants, Arabidopsis (Arabidopsis thaliana) and Brachypodium (Brachypodium distachyon). We improved myosin gene models and found that myosin genes undergo alternative splicing. We experimentally validated the gene models for Arabidopsis myosin XI-K, which plays the principal role in cell interior dynamics, as well as for its Brachypodium ortholog. We showed that the Arabidopsis gene dubbed HDK (for headless derivative of myosin XI-K), which emerged through a partial duplication of the XI-K gene, is developmentally regulated. A gene with similar architecture was also found in Brachypodium. Our analyses revealed two predominant patterns of myosin gene expression, namely pollen/stamen-specific and ubiquitous expression throughout the plant. We also found that several myosins XI can be rhythmically expressed. Phylogenetic reconstructions indicate that the last common ancestor of the angiosperms possessed two myosins VIII and five myosins XI, many of which underwent additional lineage-specific duplications.
BT - Plant physiology
C1 - http://www.ncbi.nlm.nih.gov/pubmed/21233331?dopt=Abstract
DA - 2011 Mar
IS - 3
J2 - Plant Physiol.
LA - eng
N2 - Plants possess two myosin classes, VIII and XI. The myosins XI are implicated in organelle transport, filamentous actin organization, and cell and plant growth. Due to the large size of myosin gene families, knowledge of these molecular motors remains patchy. Using deep transcriptome sequencing and bioinformatics, we systematically investigated myosin genes in two model plants, Arabidopsis (Arabidopsis thaliana) and Brachypodium (Brachypodium distachyon). We improved myosin gene models and found that myosin genes undergo alternative splicing. We experimentally validated the gene models for Arabidopsis myosin XI-K, which plays the principal role in cell interior dynamics, as well as for its Brachypodium ortholog. We showed that the Arabidopsis gene dubbed HDK (for headless derivative of myosin XI-K), which emerged through a partial duplication of the XI-K gene, is developmentally regulated. A gene with similar architecture was also found in Brachypodium. Our analyses revealed two predominant patterns of myosin gene expression, namely pollen/stamen-specific and ubiquitous expression throughout the plant. We also found that several myosins XI can be rhythmically expressed. Phylogenetic reconstructions indicate that the last common ancestor of the angiosperms possessed two myosins VIII and five myosins XI, many of which underwent additional lineage-specific duplications.
PY - 2011
SP - 1191
EP - 204
T2 - Plant physiology
TI - Expression, splicing, and evolution of the myosin gene family in plants.
VL - 155
SN - 1532-2548
ER -