TY - JOUR
KW - Amino Acid Sequence
KW - Antibodies
KW - Cell Nucleus
KW - Chloroplasts
KW - DNA, Chloroplast
KW - DNA-Directed RNA Polymerases
KW - Escherichia coli
KW - Gene Expression Regulation, Plant
KW - Genes, Plant
KW - Mitochondria
KW - Plant Proteins
KW - Promoter Regions, Genetic
KW - Protein Transport
KW - Sigma Factor
KW - Substrate Specificity
KW - Terminology as Topic
KW - Transcription, Genetic
KW - Zea mays
AU - Thomas Beardslee
AU - Sanchita Roy-Chowdhury
AU - Pankaj Jaiswal
AU - Laurence Buhot
AU - Silva Lerbs-Mache
AU - David Stern
AU - Lori Allison
AB - Plants contain nuclear gene families that encode proteins related to the principal sigma factors of eubacteria. As sigma factors function in transcription, the plant proteins have been presumed or demonstrated to associate with the eubacteria-like RNA polymerase of chloroplasts. In maize, five sig cDNA sequences have been reported, and four of the products are present in plastids as predicted. However, in vitro chloroplast import assays and computer algorithms gave ambiguous results with the fifth protein, ZmSig2B. Unlike the other maize sigma factors, ZmSig2B is expressed throughout developing seedling leaves, as well as in roots and etiolated tissues. To determine the subcellular location of ZmSig2B, we have now used immunoblot assays to show that it co-purifies with both mitochondria and plastids. Its NH2-terminal 153 amino acids, translationally fused to green fluorescent protein (GFP), targeted GFP to chloroplasts and mitochondria in bombarded maize leaves. A putative role for ZmSig2B in mitochondrial transcription is supported by its presence in a maize mitochondrial transcription extract. ZmSig2B also exhibits the expected properties of a chloroplast sigma factor: recombinant ZmSig2B binds to a chloroplast promoter and initiates transcription in vitro when combined with Escherichia coli core RNA polymerase. Therefore ZmSig2B is an unusual nucleus-encoded sigma factor that appears to function in both chloroplasts and mitochondria.
BT - The Plant journal : for cell and molecular biology
C1 - http://www.ncbi.nlm.nih.gov/pubmed/12121449?dopt=Abstract
DA - 2002 Jul
IS - 2
J2 - Plant J.
LA - eng
N2 - Plants contain nuclear gene families that encode proteins related to the principal sigma factors of eubacteria. As sigma factors function in transcription, the plant proteins have been presumed or demonstrated to associate with the eubacteria-like RNA polymerase of chloroplasts. In maize, five sig cDNA sequences have been reported, and four of the products are present in plastids as predicted. However, in vitro chloroplast import assays and computer algorithms gave ambiguous results with the fifth protein, ZmSig2B. Unlike the other maize sigma factors, ZmSig2B is expressed throughout developing seedling leaves, as well as in roots and etiolated tissues. To determine the subcellular location of ZmSig2B, we have now used immunoblot assays to show that it co-purifies with both mitochondria and plastids. Its NH2-terminal 153 amino acids, translationally fused to green fluorescent protein (GFP), targeted GFP to chloroplasts and mitochondria in bombarded maize leaves. A putative role for ZmSig2B in mitochondrial transcription is supported by its presence in a maize mitochondrial transcription extract. ZmSig2B also exhibits the expected properties of a chloroplast sigma factor: recombinant ZmSig2B binds to a chloroplast promoter and initiates transcription in vitro when combined with Escherichia coli core RNA polymerase. Therefore ZmSig2B is an unusual nucleus-encoded sigma factor that appears to function in both chloroplasts and mitochondria.
PY - 2002
SP - 199
EP - 209
T2 - The Plant journal : for cell and molecular biology
TI - A nucleus-encoded maize protein with sigma factor activity accumulates in mitochondria and chloroplasts.
VL - 31
SN - 0960-7412
ER -